2. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. In this video I quickly go over how to make a peripheral blood smear. Blood smears are needed for microscopic examination of the blood. Check out How to collect the blood sample by Venipuncture? Keep the same angle all the way to the end of the smear. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. After staining the slide, the examination begins. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Take one drop of venous or capillary blood. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. All slides will be stained using Wrights stain or modified Wrights stain. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. If you continue browsing the site, you agree to the use of cookies on this website. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. Finger Prick or . Preparation of Blood Smear: Collection of Sample: 1. (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. At one time, a blood smear was prepared on nearly everyone who had a CBC. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Blood collection for thick or thin blood smears . Peripheral Blood Smear Preparation. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. Place a small drop of blood, or one side about 1-2 cm from one end. I am a Medical Lab Tech, a Web Developer and Bibliophiliac. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. The most common technique of blood smear preparation is called the “wedge or push” technique. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. 6. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Most commonly blood smears are used for the Differential Leukocyte count (DLC). The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Tnx for all keep it up am lab technician from Ethiopia. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. There are many reasons why your doctor may order a blood smear. Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. of collection) Preparation of Blood Film: The slide should be clean. Place the Blood drop in the center of the glass slide. WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. Wear gloves. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. This article highlights the basic science and art behind the PBF. blood (within 1 hr. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. ⇒ Collect the blood sample from the capillary or venipuncture. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). Cover the blood film with undiluted staining solution. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. Samples with heparin are unusable for preparation of the smears. Check out How to do a Capillary blood collection? The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. Preparing the Peripheral Blood Film . Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. Save my name, email, and website in this browser for the next time I comment. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. associated with the decrease in the viscosity of blood. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. ⇒ Allow the blood smear to air-dry completely. Clean slides with 70 to 90% alcohol and allow to dry. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. The drop should be in the center line of the glass slide. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Thin smears. Start studying Preparation of Peripheral Blood Smear 3.  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. Your email address will not be published. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. Normally, … But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. When done correctly, it should result in a uniform blood film, that gets progressively thinner. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. Peripheral Smear Preparation. Such an area for examination is illustrated in the image below. 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